Protein-coding gene in the species Homo sapiens
Kinesin-associated protein 3 (KAP3 ) is a protein that in humans is encoded by the KIFAP3 gene .[ 5] [ 6] KIF3A and KIF3B or KIF3C , to form heterotrimeric kinesin-2 motor proteins. Kinesin-2 KAP subunits were initially characterized in echinoderms and mice.[ 7] [ 8]
Function The small G protein GDP dissociation stimulator (smg GDS ) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 Armadillo repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum , is phosphorylated by v-src , and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP ) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein.[ 6] [ 9]
Interactions KIFAP3 has been shown to interact with APC ,[ 10] SMC3 [ 11] RAP1GDS1 .[ 5]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000075945 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026585 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ a b Shimizu K, Kawabe H, Minami S, Honda T, Takaishi K, Shirataki H, Takai Y (Dec 1996). "SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase" . J Biol Chem . 271 (43): 27013– 7. doi :10.1074/jbc.271.43.27013 PMID 8900189 . ^ a b "Entrez Gene: KIFAP3 kinesin-associated protein 3" .^ Wedaman KP, Meyer DW, Rashid DJ, Cole DG, Scholey JM (February 1996). "Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex" . J. Cell Biol . 132 (3): 371– 80. doi :10.1083/jcb.132.3.371 . PMC 2120715 PMID 8636215 . ^ Yamazaki H, Nakata T, Okada Y, Hirokawa N (August 1996). "Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B" . Proc. Natl. Acad. Sci. U.S.A . 93 (16): 8443– 8. Bibcode :1996PNAS...93.8443Y . doi :10.1073/pnas.93.16.8443 PMC 38690 PMID 8710890 . ^ Ahmed Z, Mazumdar S, Ray K (February 2020). "Kinesin associated protein, DmKAP, binding harnesses the C-terminal ends of the Drosophila kinesin-2 stalk heterodimer" . Biochem Biophys Res Commun . 522 (2): 506– 511. doi :10.1016/j.bbrc.2019.11.111 . PMC 8407544 PMID 31784087 . ^ Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, Akiyama T (April 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nat. Cell Biol . 4 (4): 323– 7. doi :10.1038/ncb779 . PMID 11912492 . S2CID 10745049 . ^ Shimizu K, Shirataki H, Honda T, Minami S, Takai Y (March 1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide" . J. Biol. Chem . 273 (12): 6591– 4. doi :10.1074/jbc.273.12.6591 PMID 9506951 .
Further reading
Nagata K, Puls A, Futter C, et al. (1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3" . EMBO J . 17 (1): 149– 58. doi :10.1093/emboj/17.1.149 . PMC 1170366 PMID 9427749 . Shimizu K, Shirataki H, Honda T, et al. (1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide" . J. Biol. Chem . 273 (12): 6591– 4. doi :10.1074/jbc.273.12.6591 PMID 9506951 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899– 903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40– 5. doi :10.1038/ng1285 PMID 14702039 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121– 7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Khanna H, Hurd TW, Lillo C, et al. (2005). "RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC1, SMC3, and microtubule transport proteins" . J. Biol. Chem . 280 (39): 33580– 7. doi :10.1074/jbc.M505827200 PMC 1249479 PMID 16043481 . Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature . 437 (7062): 1173– 8. Bibcode :2005Natur.437.1173R . doi :10.1038/nature04209 . PMID 16189514 . S2CID 4427026 . Brown CL, Maier KC, Stauber T, et al. (2006). "Kinesin-2 is a motor for late endosomes and lysosomes" . Traffic . 6 (12): 1114– 24. doi :10.1111/j.1600-0854.2005.00347.x PMID 16262723 . Haraguchi K, Hayashi T, Jimbo T, et al. (2006). "Role of the kinesin-2 family protein, KIF3, during mitosis" . J. Biol. Chem . 281 (7): 4094– 9. doi :10.1074/jbc.M507028200 PMID 16298999 . Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1" . Nature . 441 (7091): 315– 21. Bibcode :2006Natur.441..315G . doi :10.1038/nature04727 PMID 16710414 . Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration" . Cell . 125 (4): 801– 14. doi :10.1016/j.cell.2006.03.032 PMID 16713569 . S2CID 13709685 . Kuraguchi M, Wang XP, Bronson RT, et al. (2006). "Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus" . PLOS Genet . 2 (9): e146. doi :10.1371/journal.pgen.0020146 PMC 1564426 PMID 17002498 . Stauber T, Simpson JC, Pepperkok R, Vernos I (2007). "A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex" . Curr. Biol . 16 (22): 2245– 51. doi :10.1016/j.cub.2006.09.060 PMID 17113389 . S2CID 9739953 .
