Protein-coding gene in the species Homo sapiens
Death-associated protein kinase 2 is an enzyme that in humans is encoded by the DAPK2 gene .[ 5] [ 6]
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death . Gene overexpression induces cell apoptosis . It uses multiple polyadenylation sites.[ 6]
The DAPK2 mRNA may undergo alternative splicing to produce a DAPK3 -like encoding transcript.[ 7]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000035664 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032380 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S (Jun 1999). "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity". Oncogene . 18 (23): 3471– 80. doi :10.1038/sj.onc.1202701 . PMID 10376525 . S2CID 19035227 . ^ a b "Entrez Gene: DAPK2 death-associated protein kinase 2" .^ Shoval Y, Berissi H, Kimchi A, Pietrokovski S (2011). "New Modularity of DAP-Kinases: Alternative Splicing of the DRP-1 Gene Produces a ZIPk-Like Isoform" . PLOS ONE . 6 (2): e17344. Bibcode :2011PLoSO...617344S . doi :10.1371/journal.pone.0017344 PMC 3050894 PMID 21408167 .
Further reading
Inbal B, Shani G, Cohen O, et al. (2000). "Death-Associated Protein Kinase-Related Protein 1, a Novel Serine/Threonine Kinase Involved in Apoptosis" . Mol. Cell. Biol . 20 (3): 1044– 54. doi :10.1128/MCB.20.3.1044-1054.2000 . PMC 85221 PMID 10629061 . Shani G, Henis-Korenblit S, Jona G, et al. (2001). "Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding" . EMBO J . 20 (5): 1099– 113. doi :10.1093/emboj/20.5.1099 . PMC 145456 PMID 11230133 . Wong TS, Chang HW, Tang KC, et al. (2002). "High frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients". Clin. Cancer Res . 8 (2): 433– 7. PMID 11839660 . Chan MW, Chan LW, Tang NL, et al. (2002). "Hypermethylation of multiple genes in tumor tissues and voided urine in urinary bladder cancer patients". Clin. Cancer Res . 8 (2): 464– 70. PMID 11839665 . Inbal B, Bialik S, Sabanay I, et al. (2002). "DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death" . J. Cell Biol . 157 (3): 455– 68. doi :10.1083/jcb.200109094 . PMC 2173279 PMID 11980920 . Satoh A, Toyota M, Itoh F, et al. (2002). "DNA methylation and histone deacetylation associated with silencing DAP kinase gene expression in colorectal and gastric cancers" . Br. J. Cancer . 86 (11): 1817– 23. doi :10.1038/sj.bjc.6600319 . PMC 2375414 PMID 12087472 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899– 903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121– 7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Barrios-Rodiles M, Brown KR, Ozdamar B, et al. (2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science . 307 (5715): 1621– 5. Bibcode :2005Sci...307.1621B . doi :10.1126/science.1105776 . PMID 15761153 . S2CID 39457788 . Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks" . Cell . 127 (3): 635– 48. doi :10.1016/j.cell.2006.09.026 PMID 17081983 . Rizzi M, Tschan MP, Britschgi C, et al. (2007). "The death-associated protein kinase 2 is up-regulated during normal myeloid differentiation and enhances neutrophil maturation in myeloid leukemic cells" . J. Leukoc. Biol . 81 (6): 1599– 608. doi :10.1189/jlb.0606400 PMID 17347302 .
PDB gallery
1wmk : Human death-associated kinase DRP-1, mutant S308D d40
1z9x : Human DRP-1 kinase, W305S S308A D40 mutant, crystal form with 3 monomers in the asymmetric unit
1zws : Crystal structure of the catalytic domain of human DRP-1 kinase
2a27 : Human DRP-1 kinase, W305S S308A D40 mutant, crystal form with 8 monomers in the asymmetric unit
2a2a : High-resolution crystallographic analysis of the autoinhibited conformation of a human death-associated protein kinase
2cke : HUMAN DEATH-ASSOCIATED DRP-1 KINASE IN COMPLEX WITH INHIBITOR
Activity Regulation Classification Kinetics Types
