Act of Murder may refer to:

• An Act of Murder, a 1948 American film noir directed by Michael Gordon
• Act of Murder (film), a 1964 British crime drama film directed by Alan Bridges
• Act of Murder, a 1993 documentary film by Shirley Horrocks
• Act of Murder (novel), a 2010 novel by Alan Wright
• An Act of Murder, a work of interactive fiction by Christopher Huang, nominated for multiple 2007 XYZZY Awards

• Homicide
• Murder
• UK Acts of Parliament
  • Murder Act 1751
  • Homicide Act 1957
  • Murder (Abolition of Death Penalty) Act 1965