Ann Walker or Anne Walker may refer to:

• Ann Walker (landowner) (1803–1854), Yorkshire landowner, partner of Anne Lister
• Anne Walker (architectural historian) (born 1973), American architectural historian and author
• Anne Walker (artist) (born 1933), painter and printmaker
• Anne Walker (astronomer) (1863–1940), British astronomer
• Anne Weightman Walker (1844–1932), American philanthropist

• Annie Walker (disambiguation)
• Anna Walker (disambiguation)
• Walker (surname)