Ace in the Hole may refer to:

• Ace in the Hole (album), an album by Elvin Bishop
• Ace in the Hole Band, the backup band for George Strait
• "Ace in the Hole" (Cole Porter song)
• "Ace in the Hole" (George Strait song) written by Dennis Adkins
• "Ace in the Hole", a song by Paul Simon on the album One-Trick Pony
• "Ace in the Hole", a song composed in 1909 by James Dempsey and George Mitchell, and recorded by many artists including Bobby Darin and Johnny Mercer on Two of a Kind

• Ace in the Hole (1942 film), an animated cartoon short subject
• Ace in the Hole (1951 film), a film starring Kirk Douglas
• Ace in the Hole (anthology), edited by George R. R. Martin
• Ace in the Hole, a 2009 sitcom pilot starring Adam Carolla
• Having an ace as a hole card in poker
• That Old Ace in the Hole, a 2002 novel by Annie Proulx
• The Chip Chip Hooray creation myth