Protein-coding gene in the species Homo sapiens
Ubiquitin-conjugating enzyme E2 D1 is a protein that in humans is encoded by the UBE2D1 gene .[ 5] [ 6] [ 7]
Function The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.[ 7]
Interactions UBE2D1 has been shown to interact with:
References
^ a b c GRCh38: Ensembl release 89: ENSG00000072401 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000019927 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Robinson PA, Leek JP, Ardley HC, Thompson J, Rose SA, Markham AF (March 1999). "Assignment of UBE2D1 to human chromosome bands 10q11.2→q21 by in situ hybridization". Cytogenet Cell Genet . 83 (3–4): 247–8. doi :10.1159/000015195 . PMID 10072594 . S2CID 3059748 . ^ Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM (January 1996). "Identification of a family of closely related human ubiquitin conjugating enzymes" . J Biol Chem . 270 (51): 30408–14. doi :10.1074/jbc.270.51.30408 PMID 8530467 . ^ a b "Entrez Gene: UBE2D1 ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)" .^ a b Mallery DL, Vandenberg CJ, Hiom K (Dec 2002). "Activation of the E3 ligase function of the BRCA1/BARD1 complex by polyubiquitin chains" . EMBO J . 21 (24): 6755–62. doi :10.1093/emboj/cdf691 . PMC 139111 PMID 12485996 . ^ a b Kentsis A, Gordon RE, Borden KL (November 2002). "Control of biochemical reactions through supramolecular RING domain self-assembly" . Proc. Natl. Acad. Sci. U.S.A . 99 (24): 15404–9. Bibcode :2002PNAS...9915404K . doi :10.1073/pnas.202608799 PMC 137729 PMID 12438698 . ^ a b Chen A, Kleiman FE, Manley JL, Ouchi T, Pan ZQ (June 2002). "Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase" . J. Biol. Chem . 277 (24): 22085–92. doi :10.1074/jbc.M201252200 PMID 11927591 . ^ a b Dong Y, Hakimi MA, Chen X, Kumaraswamy E, Cooch NS, Godwin AK, Shiekhattar R (November 2003). "Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair" . Mol. Cell . 12 (5): 1087–99. doi :10.1016/s1097-2765(03)00424-6 PMID 14636569 . ^ a b Sato K, Hayami R, Wu W, Nishikawa T, Nishikawa H, Okuda Y, Ogata H, Fukuda M, Ohta T (July 2004). "Nucleophosmin/B23 is a candidate substrate for the BRCA1-BARD1 ubiquitin ligase" . J. Biol. Chem . 279 (30): 30919–22. doi :10.1074/jbc.C400169200 PMID 15184379 . ^ a b Wu-Baer F, Lagrazon K, Yuan W, Baer R (September 2003). "The BRCA1/BARD1 heterodimer assembles polyubiquitin chains through an unconventional linkage involving lysine residue K6 of ubiquitin" . J. Biol. Chem . 278 (37): 34743–6. doi :10.1074/jbc.C300249200 PMID 12890688 . ^ a b Vandenberg CJ, Gergely F, Ong CY, Pace P, Mallery DL, Hiom K, Patel KJ (July 2003). "BRCA1-independent ubiquitination of FANCD2" . Mol. Cell . 12 (1): 247–54. doi :10.1016/s1097-2765(03)00281-8 PMID 12887909 . ^ a b Hashizume R, Fukuda M, Maeda I, Nishikawa H, Oyake D, Yabuki Y, Ogata H, Ohta T (May 2001). "The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation" . J. Biol. Chem . 276 (18): 14537–40. doi :10.1074/jbc.C000881200 PMID 11278247 . ^ Brzovic PS, Keeffe JR, Nishikawa H, Miyamoto K, Fox D, Fukuda M, Ohta T, Klevit R (May 2003). "Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex" . Proc. Natl. Acad. Sci. U.S.A . 100 (10): 5646–51. Bibcode :2003PNAS..100.5646B . doi :10.1073/pnas.0836054100 PMC 156255 PMID 12732733 . ^ Nishikawa H, Ooka S, Sato K, Arima K, Okamoto J, Klevit RE, Fukuda M, Ohta T (February 2004). "Mass spectrometric and mutational analyses reveal Lys-6-linked polyubiquitin chains catalyzed by BRCA1-BARD1 ubiquitin ligase" . J. Biol. Chem . 279 (6): 3916–24. doi :10.1074/jbc.M308540200 PMID 14638690 . ^ Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M (February 1996). "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5" . J. Biol. Chem . 271 (5): 2795–800. doi :10.1074/jbc.271.5.2795 PMID 8576257 . ^ Nuber U, Scheffner M (March 1999). "Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction" . J. Biol. Chem . 274 (11): 7576–82. doi :10.1074/jbc.274.11.7576 PMID 10066826 .
Further reading
Scheffner M, Huibregtse JM, Howley PM (1994). "Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53" . Proc. Natl. Acad. Sci. U.S.A . 91 (19): 8797–801. Bibcode :1994PNAS...91.8797S . doi :10.1073/pnas.91.19.8797 PMC 44693 PMID 8090726 . Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery" . Genome Res . 6 (9): 791–806. doi :10.1101/gr.6.9.791 PMID 8889548 . Hatakeyama S, Jensen JP, Weissman AM (1997). "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases" . J. Biol. Chem . 272 (24): 15085–92. doi :10.1074/jbc.272.24.15085 PMID 9182527 . Gutierrez JA, Yu J, Rivera S, Wessling-Resnick M (1997). "Functional Expression Cloning and Characterization of SFT, a Stimulator of Fe Transport" . J. Cell Biol . 139 (4): 895–905. doi :10.1083/jcb.139.4.895 . PMC 2139974 PMID 9362508 . Gutierrez JA, Yu J, Wessling-Resnick M (1999). "Characterization and chromosomal mapping of the human gene for SFT, a stimulator of Fe transport". Biochem. Biophys. Res. Commun . 253 (3): 739–42. doi :10.1006/bbrc.1998.9836 . PMID 9918797 . Nuber U, Scheffner M (1999). "Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction" . J. Biol. Chem . 274 (11): 7576–82. doi :10.1074/jbc.274.11.7576 PMID 10066826 . Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW (2000). "Activation of HIF1α ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex" . Proc. Natl. Acad. Sci. U.S.A . 97 (19): 10430–5. Bibcode :2000PNAS...9710430K . doi :10.1073/pnas.190332597 PMC 27041 PMID 10973499 . Pringa E, Martinez-Noel G, Muller U, Harbers K (2001). "Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes" . J. Biol. Chem . 276 (22): 19617–23. doi :10.1074/jbc.M100192200 PMID 11274149 . Hashizume R, Fukuda M, Maeda I, Nishikawa H, Oyake D, Yabuki Y, Ogata H, Ohta T (2001). "The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation" . J. Biol. Chem . 276 (18): 14537–40. doi :10.1074/jbc.C000881200 PMID 11278247 . Matsuzawa SI, Reed JC (2001). "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses" . Mol. Cell . 7 (5): 915–26. doi :10.1016/S1097-2765(01)00242-8 PMID 11389839 . Jiang J, Ballinger CA, Wu Y, Dai Q, Cyr DM, Höhfeld J, Patterson C (2001). "CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation" . J. Biol. Chem . 276 (46): 42938–44. doi :10.1074/jbc.M101968200 PMID 11557750 . Chen A, Kleiman FE, Manley JL, Ouchi T, Pan ZQ (2002). "Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase" . J. Biol. Chem . 277 (24): 22085–92. doi :10.1074/jbc.M201252200 PMID 11927591 . Badciong JC, Haas AL (2003). "MdmX is a RING finger ubiquitin ligase capable of synergistically enhancing Mdm2 ubiquitination" . J. Biol. Chem . 277 (51): 49668–75. doi :10.1074/jbc.M208593200 PMID 12393902 . Kentsis A, Gordon RE, Borden KL (2003). "Control of biochemical reactions through supramolecular RING domain self-assembly" . Proc. Natl. Acad. Sci. U.S.A . 99 (24): 15404–9. Bibcode :2002PNAS...9915404K . doi :10.1073/pnas.202608799 PMC 137729 PMID 12438698 . Gehrke SG, Riedel HD, Herrmann T, Hadaschik B, Bents K, Veltkamp C, Stremmel W (2003). "UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis" . Blood . 101 (8): 3288–93. doi :10.1182/blood-2002-07-2192 PMID 12480712 . Mallery DL, Vandenberg CJ, Hiom K (2004). "Activation of the E3 ligase function of the BRCA1/BARD1 complex by polyubiquitin chains" . EMBO J . 21 (24): 6755–62. doi :10.1093/emboj/cdf691 . PMC 139111 PMID 12485996 . Takeyama K, Aguiar RC, Gu L, He C, Freeman GJ, Kutok JL, Aster JC, Shipp MA (2003). "The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity" . J. Biol. Chem . 278 (24): 21930–7. doi :10.1074/jbc.M301157200 PMID 12670957 .
