Tooley is a surname that may refer to:

• Bert Tooley (1886–1976), American professional baseball player
• James Tooley (b. 1959), British professor of education policy at Newcastle University
• John Tooley (1924–2020), English musical administrator
• John Tooley (weightlifter) (born 1897), British Olympic weightlifter
• Léon Tooley (b. 1972), British novelist
• Mark Tooley (b. 1965), American conservative magazine editor and think-tank president
• Max Tooley (born 1998), American football player
• Michael Tooley, American philosopher
• Nicholas Tooley (1583–1623), English Renaissance actor
• Richard Tooley (1820–1910), Canadian farmer and provincial assembly legislator from Ontario
• S.D. Tooley (contemporary), American novelist of mystery and urban fantasy

It may also refer to:

• Tooley Street, London street in the Borough of Southwark
• 1861 Tooley Street fire, a fire on Tooley Street

• Toley, fictional character played by Ron Hayes in the television western Two Faces West 1961 episode "Music Box"
• Albert Toley (1855-1924), founder and first president of Brent Valley Golf Club in Hanwell, London, UK
• George Toley (1916-2008), American collegiate coach