Toole is a surname that may refer to:

• F.X. Toole (1930–2002), American boxing trainer and author
• John Kennedy Toole (1937–1969), American novelist
• Annette O'Toole (born Toole; 1952), American actress, dancer, and singer-songwriter
• John Lawrence Toole (1830–1906), English comic actor and theatrical producer
• Joseph Toole (1851–1929), American politician from Montana; governor of Montana 1889–93
• K. Ross Toole (1920–1981), American historian and author
• Melinda Toole (contemporary), American beauty queen; Miss Alabama 2006
• Ottis Toole (1947–1996), American serial killer
• Steve Toole (1859–1919), American professional baseball player
• Zula Brown Toole (1868–1947), American newspaper publisher
• Paul Toole (1970- ), Deputy Premier of New South Wales

• Tool (disambiguation)
• Toole County, Montana, a county in the United States
• Tooley, a surname
• Tulle, a commune in central France