SYTOX
SYTOX (also known as SYTOX Green) is a high-affinity nucleic acid stain developed by biotechnology company Molecular Probes. Because the stain only penetrates cells with compromised plasma membranes, it can be used to investigate antibacterial mechanism of action[1] and confirm loss of bacterial viability.[2][3] There have, however, been studies which confirm the use of SYTOX dyes for live cell imaging of bacteria.[4]
A comprehensive Study of Thiazole-Orange-Based DNA Dyes were published, involving SYBR Safe, SYBR Green, PicoGreen, SYTO-16, SYTO-9 and a new derivative TOPhBu. Here, the synthesis of these compounds are published and characterised spectro-scopically to quantify their fluorescence enhancement upon binding to double-stranded DNA. The ability of the dyes to detect DNA at low concentrations was evaluated using two new metrics, absolute fluorescence enhancement (AFE) and relativefluorescence enhancement (RFE). They were tested in qPCR experiments showing some important differences in the sensitivity and qPCR efficiency, which facilitate the DNA marker selection for analytical purposes.[5]
References
- ^ Lee H, Woo ER, Lee DG (July 2015). "Glochidioboside Kills Pathogenic Bacteria by Membrane Perturbation". Current Microbiology. 71 (1): 1–7. doi:10.1007/s00284-015-0807-9. PMID 25820208. S2CID 14011127.
- ^ Gaforio JJ, Serrano MJ, Ortega E, Algarra I, Alvarez de Cienfuegos G (June 2002). "Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis". Cytometry. 48 (2): 93–96. doi:10.1002/cyto.10107. PMID 12116370.
- ^ Lebaron P, Catala P, Parthuisot N (July 1998). "Effectiveness of SYTOX Green stain for bacterial viability assessment". Applied and Environmental Microbiology. 64 (7): 2697–2700. Bibcode:1998ApEnM..64.2697L. doi:10.1128/AEM.64.7.2697-2700.1998. PMC 106447. PMID 9647851.
- ^ Bakshi S, Choi H, Rangarajan N, Barns KJ, Bratton BP, Weisshaar JC (August 2014). Parales RE (ed.). "Nonperturbative imaging of nucleoid morphology in live bacterial cells during an antimicrobial peptide attack". Applied and Environmental Microbiology. 80 (16): 4977–4986. Bibcode:2014ApEnM..80.4977B. doi:10.1128/AEM.00989-14. PMC 4135745. PMID 24907320.
- ^ Domahidy F, Kovács B, Cseri L, Katona G, Rózsa B, Mucsi Z, Kovács E, Kovacs E (2024-06-19). "Comprehensive Study of Thiazole-Orange-Based DNA Dyes". ChemPhotoChem: e202400080. doi:10.1002/cptc.202400080. ISSN 2367-0932.