Queen B may refer to:

• Queen Bee, a nickname given in the 1990's to former Junior Mafia member, Lil Kim
• Queen B/Bey/Bee, a nickname for Beyonce
• Queen B, a nickname for the former queen Beatrix of the Netherlands
• Queen B, a nickname for South African TV and Radio personality Bonang Matheba
• Queen B, a nickname for Blair Waldorf in Gossip Girl

• "Queen B.", a 2007 song by Puscifer

• Queen B, a 2005 TV movie starring Alicia Silverstone
• "Queen B.", an episode of Popular
• "Queen B." (Arrested Development), an episode of Arrested Development

• Queen bee (disambiguation)