Pusey may refer to:

• Caleb Pusey (c. 1650–1727), friend and business partner of William Penn
• Chris Pusey (born 1965), Canadian ice hockey player
• Edward Bouverie Pusey (1800–1882), English churchman
• Ernest Pusey (1895–2006), World War I veteran and oldest living person in Florida
• Frederick Taylor Pusey (1872–1936), member of the Pennsylvania House of Representatives
• Jacqueline Pusey (born 1959), Jamaican sprint athlete
• Jason Pusey (born 1989), Gibraltarian footballer
• Joshua Pusey (1842–1906), American inventor of the paper matchbook
• Mavis Pusey (1928–2019), Jamaican-born painter
• Merlo J. Pusey (1902–1985), American biographer
• Nathan Pusey (1907–2001), American educator and 24th president of Harvard University (1953–1971)
• Philip Pusey (1799–1855), English agriculturalist and Member of Parliament
• Philip E. Pusey (1830–1880), English Aramaicist
• Peter Pusey (born 1942), British physicist
• Stephen Pusey (born 1952), British-born artist
• William A. Pusey (1865–1940), American physician and past president of the American Medical Association.
• William Henry Mills Pusey (1826–1900), U.S. Representative

• Pusey, Haute-Saône, a commune of the Haute-Saône département in France
• Pusey, Ontario, a community in the township of Highlands East, Ontario, Canada
• Pusey, Oxfordshire, a small village with stately home, in Oxfordshire, England
• Pewsey, a village in Wiltshire, England

• Pusey and Jones, a former American shipbuilder and industrial-equipment manufacturer
• Pusey House, Oxford, an Anglican religious institution
• Pusey Street, Oxford, England