The Orto Botanico dell'Università di Lecce (2 hectares), also known as the Orto Botanico di Lecce, is a botanical garden operated by the University of Lecce and located at Via Provinciale Lecce-Monteroni, 73100, Lecce, Apulia, Italy.

Although Lecce's earliest botanical garden dates to 1814, it began an irreversible decline in 1866 after its acquisition by the province, and today's garden is a separate undertaking begun in 1994 by the university's biology department. It currently contains several hundred species.

• List of botanical gardens in Italy

• Botanical Garden University of Salento (in Italian)
• S. Marchiori, "L'Orto Botanico di Lecce", Museol. Sci., XII (1-2): 143–148, 1995.
• Gruppo Italiano per la Ricerca sulle Orchidee Spontanee (GIROS), Notiziario per i Soci, September 2002, no 21. PDF

40°20′N 18°10′E / 40.333°N 18.167°E / 40.333; 18.167