Ophis is Greek for "serpent", and may refer to:

• The constellation Serpens
• Ophis (Pontus), a town of ancient Pontus, now in Turkey
• Ophis (river), a river of ancient Anatolia
• Serpent (Bible), a figure in the Hebrew bible
• SS Ophis, a cargo ship built in 1919 and which was sunk in 1942 when named Empire Antelope
• Ophis (High School DxD), a character in the light novel series High School DxD

• Apep