Margaret Stewart or Stuart may refer to:

• Margaret Stewart, Countess of Angus (died 1417), wife of Thomas, Earl of Mar; mother of George Douglas, 1st Earl of Angus
• Margaret Stewart, Dauphine of France (1424–1445), princess of Scotland; Dauphine of France as wife to future Louis XI
• Margaret Stewart (born c. 1455), daughter of James II of Scotland and niece of the Dauphine
• Margaret Douglas (1515–1578), married name Margaret Stewart, countess of Lennox and half-sister of James V of Scotland
• Margaret Tudor (1489–1541), queen and regent of Scotland, wife of James IV of Scotland and sister of Henry VIII of England
• Margaret Stuart (1598–1600), daughter of James VI.
• Margaret of Denmark, Queen of Scotland (1456–1486), daughter of King Christian I of Denmark and wife of James III, King of Scots
• Margaret Howard, Countess of Nottingham (c. 1591–1639), née Margaret Stewart
• Margaret Stewart, Mistress of Ochiltree (died 1627), courtier in the household of Anne of Denmark
• Margaret Stewart, Lady Gordon (born 1498), daughter of James IV of Scotland and Margaret Drummond

• Margie Stewart (1919–2012), American model
• Margaret Stuart (poet) (1889–1963), British poet and writer
• Margaret Stuart (athlete) (1934–1999), New Zealand sprinter and hurdler
• Margaret Stewart (herpetologist) (1927–2006), American herpetologist
• Margaret E. C. Stewart (1907–1986), Scottish archaeologist

• Peggy Stewart (disambiguation)