Ladies and Gentlemen may refer to:

• Ladies and gentlemen (salutation), a common introductory phrase

• Ladies & Gentlemen (2015 film), an Indian Telugu-language film
• Ladies & Gentlemen (TV series), a 2021 Bangladeshi drama series
• Ladies and Gentlemen (Comedy Showcase), a 2007 one-off British comedy special
• "Ladies and Gentlemen" (Master of None), a 2015 television episode

• Ladies and Gentlemen (Lou Bega album), 2001
• Ladies and Gentlemen (Marcia Hines album), 1977
• Ladies & Gentlemen: The Best of George Michael, 1998
• Ladies & Gentlemen: The Songs of George Michael, by Anthony Callea, 2014
• Ladies and Gentlemen: Barenaked Ladies and The Persuasions, 2017
• Ladies and Gentlemen... Mr. B.B. King, 2012
• Ladies and Gentlemen... The Bangles!, 2014
• Ladies and Gentlemen... the Grateful Dead, 2000
• Ladies & Gentlemen, by the Infamous Stringdusters, 2016

• "Ladies and Gentlemen" (song), by Saliva, 2006
• "Ladies and Gentlemen", by City and Colour from The Hurry and the Harm, 2013
• "Ladies & Gentlemen", by Short Stack from Stack Is the New Black, 2009

• Ladies & Gentlemen: The Best of George Michael (video), 1999
• Ladies and Gentlemen: The Rolling Stones, a 1974 concert movie

• Ladies and Gentlemen (play), a 1939 play by Charles MacArthur and Ben Hecht
• Ladies and Gentlemen (Warhol series) (1975), a series of paintings by Andy Warhol
• Ladies & Gentlemen, the company at the centre of the 2009 Enten controversy

• All pages with titles beginning with Ladies and Gentlemen
• All pages with titles beginning with Ladies & Gentlemen
• Ladies and Gentleman, a 2013 Indian Malayalam-language film
• Lady and Gent, a 1932 American film
• Lady & Gentlemen, a 2011 album by LeAnn Rimes
• "Deviyon aur Sajjano" (lit. 'Ladies and Gentlemen'), a Hindi catchphrase by Amitabh Bachchan in the Indian game show Who Wants to Be a Millionaire?