John Guthrie may refer to:

• John Guthrie (bishop of Ross) (died c. 1494), Scottish Roman Catholic bishop
• John Guthrie (bishop of Moray) (died 1649), Church of Scotland bishop
• John Guthrie (cricketer) (1795–1865), English cricketer
• John B. Guthrie (1807–1885), mayor of Pittsburgh, Pennsylvania
• John R. Guthrie (1921–2009), U.S. Army general
• John Guthrie (novelist) (1905–1955), author from New Zealand
• John Guthrie (politician), British political activist
• John Guthrie (basketball coach), former head basketball coach of the Georgia Bulldogs

• Jack Guthrie (1915–1948), musician