Holguin or Holguín may refer to:

• Holguín, a city in Cuba
• Holguín Province in Cuba

• Jeffrey Holguin (born 1978), an American sport shooter
• Brian Holguin, American comic book writer
• Jorge Holguín (1848–1928), a Colombian politician and military officer, and two time Acting President of Colombia
• Jose L. Holguin (1921–1994), a United States Air Force Colonel
• Juan Ignacio Larrea Holguín (1927–2006), archbishop of Guayaquil
• María Ángela Holguín (born in 1963), the Minister of Foreign Affairs of Colombia
• Pedro Álvarez Holguín (1490–1542), a Spanish nobleman, military and Conquistador of Perú

• Holguín (baseball), a Cuban team
• FC Holguín, a Cuban football team