GNU is a Unix-like computer operating system developed by the GNU Project.

GNU or gnu may also refer to:

• Gnu, or wildebeests, a genus of antelopes
• GNU Project, a free software, mass collaboration project
• 9965 GNU, an asteroid

• government of national unity, a coalition government during a national emergency
• Government of National Unity (Libya), a transitional unitary government during 2021
• Government of National Unity (South Africa), a constitutional arrangement
• Grand National Union of Kenya, a Kenyan political party
• Great Northern Union, a US men's barbershop chorus
• Gyeongsang National University, a university in South Korea

• Gnau language, by ISO 639-3 language code
• Gnu Snowboards, produced by Mervin Manufacturing in the US
• Gnu Butte, a butte in British Columbia, Canada
• Goodnews Airport, Alaska, US, by IATA and FAA LID codes
• Sopwith Gnu, a 1910s British touring biplane
• "The Gnu", a song by Flanders and Swann
• A fairy chess piece used in Wildebeest chess
• The Gnus, an upper-school student newspaper of Sandy Spring Friends School
• The Gnu Theatre, a Los Angeles theatre designed and built by Jeff Seymour
• The Gnu, a pub in North Newbald, England
• Gary Gnu, fictional character in The Great Space Coaster
• A message on the clacks in Terry Pratchett's Discworld fantasy world that is repeated indefinitely along the line in memory of someone.

• Gnu goat, or Takin, a goat-antelope found in the eastern Himalayas
• Gnu High, an album by Canadian musician Kenny Wheeler
• GNU license (disambiguation)
• All pages with titles beginning with GNU
• All pages with titles containing GNU