Daddy longlegs or daddy long legs may refer to:

• Opiliones or harvestmen, an order of arachnids
• Pholcidae or cellar spiders, a family of spiders
• Crane fly, a family of insects in the order Diptera

• Stylidium divaricatum, a species of triggerplant native to Western Australia
• Caladenia filamentosa, a species of orchid native to Eastern Australia
• Brassavola cucullata, a species of orchid native to Mexico

• Daddy-Long-Legs (novel), a novel by Jean Webster
• Daddy-Long-Legs (play), a play by Jean Webster, adapted from her novel
  • Daddy-Long-Legs (1919 film), a silent comedy-drama starring Mary Pickford
  • Daddy Long Legs (1931 film), a musical starring Janet Gaynor and Warner Baxter
  • Daddy Long Legs (1938 film), a Dutch romantic comedy
  • Daddy Long Legs (1955 film), a musical starring Fred Astaire
  • Daddy-Long-Legs (2005 film), a Korean romance
  • Daddy Long Legs (musical), a 2009 musical play by John Caird and Paul Gordon
• Daddy Longlegs (2009 film), an American comedy-drama by Ben and Joshua Safdie
• "Old Father Longlegs", a nursery rhyme by Mother Goose

• Daddy Long Legs (musician), in Wolfpac and Bloodhound Gang
• Daddy Longlegs (album), by John Craigie
• "Daddy Longlegs", a 1993 song by Tumbleweed
• "Daddy Longlegs", a 2017 song by McCafferty

• Daddy Long Legs (horse) (born 2009), a Thoroughbred racehorse
• Daddy Long Legs (sculpture), a 2006 sculpture in Portland, Oregon
• Pioneer, a car on the former Brighton and Rottingdean Seashore Electric Railway