Colonial Conference may refer to:

• Colonial Conference (New Jersey), a New Jersey high school sports league
• Colonial Hills Conference, a New Jersey high school sports league
• Colonial States Athletic Conference, an NCAA Division III athletic conference
• Colonial Valley Conference, a New Jersey high school sports league
• Coastal Athletic Association – formerly known as the Colonial Athletic Association, an NCAA Division I athletic conference
• Imperial Conference (also called "Colonial Conference") meetings of heads of governments in the British Empire