Bertoni is an Italian surname. Notable people with the surname include:

• Andrea Bertoni O.S.M. (1454–1483), Roman Catholic priest and saint patron of Faenza
• Angelo Bertoni (1933–2021), member of the Queensland Legislative Assembly
• Arnoldo de Winkelried Bertoni (1878–1973), Paraguayan zoologist
• Daniel Bertoni (born 1955), former Argentine footballer
• Ferdinando Bertoni (1725–1813), Italian composer and organist
• Flaminio Bertoni (1903–1964), automobile designer
• Francesca Bertoni (born 1993), Italian female 3000 metres steeplechaser
• Gaspar Bertoni (1777–1853), Italian saint
• Letizia Bertoni (born 1937), former Italian hurdler
• Luca Bertoni (born 1992), Italian professional footballer
• Luigi Bertoni (1872–1947), Italian-born anarchist writer and typographer
• Moisés Santiago Bertoni (1857–1929), botanist known for his studies on Stevia
  • Moises Bertoni Foundation, for the protection and sustainable development of natural resources in Paraguay
• Remo Bertoni (1909–1973), Italian professional road bicycle racer
• Remo Bertoni (footballer) (1929–1993), Italian retired footballer and manager
• Sergio Bertoni (1915–1995), Italian football (soccer) player
• Xavier Bertoni (born 1988), French Freestyle skier

• 15908 Bertoni, minor planet discovered by the Kitt Peak on October 2, 1997
• Bertoni's antbird, species of bird in the family Thamnophilidae
• GDE Bertoni, trophy and medal manufacturing company
• Bertoni's antbird : species of bird in the family Thamnophilidae
• Doctor Moisés Bertoni, village in the Caazapá department of Paraguay
• Bertone