The 1999–2000 season was Ternana Calcio's 75th season in existence and third consecutive in the Serie B, the second division of Italian football. They also competed in the Coppa Italia.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply. Players may hold more than one non-FIFA nationality.

No. Pos. Nation Player — GK  ITA Daniele Balli — GK  ITA Paolo Fabbri — DF  ITA Dario Baccin — DF  ITA Cristian Stellini — DF  ITA Dimitri Birk — DF  ITA Marino D'Aloisio — DF  ITA Daniele Giannini — DF  ITA Gianluca Grava — DF  ITA Settimio Lucci — DF  ITA Simone Moretti — DF  ITA Carlo Sassarini — DF  ITA Cristian Servidei — DF  ITA Cristian Stellini — DF  ITA Carlo Teodorani

No. Pos. Nation Player — MF  ITA Luca Cavallo — MF  ITA Davide Cordone — MF  ITA Alessandro Cucciari — MF  ITA Fabrizio Fabris — MF  AUS Vince Grella — MF  ITA Riccardo Rovinelli — FW  ITA Fabio Artico — FW  ITA Francesco Baiano — FW  ITA Massimo Borgobello — FW  ITA Pierpaolo Bresciani — FW  ITA Enrico Buonocore — FW  ITA Fabrizio Miccoli — FW  ITA Giovanni Tiberi

Source:^[1]