The 1994 Iowa State Cyclones football team represented Iowa State University during the 1994 NCAA Division I-A football season. They played their home games at Cyclone Stadium in Ames, Iowa. They participated as members of the Big Eight Conference. The team was coached by head coach Jim Walden, who retired from coaching after the conclusion of the season.

Date	Time	Opponent	Site	TV	Result	Attendance
September 3	1:00 p.m.	Northern Iowa*	Cyclone Stadium Ames, IA		L 14–28	40,295
September 10	2:30 p.m.	at Iowa*	Kinnick Stadium Iowa City, IA (Battle for the Cy-Hawk Trophy)	ABC	L 9–37	70,397
September 17	1:00 p.m.	Western Michigan*	Cyclone Stadium Ames, IA		L 19–23	32,080
September 24	1:00 p.m.	Rice*	Cyclone Stadium Ames, IA		L 18–28	31,106
October 1	1:30 p.m.	at No. 21 Oklahoma	Oklahoma Memorial Stadium Norman, OK		L 6–34	65,821
October 15	1:00 p.m.	Kansas	Cyclone Stadium Ames, IA		L 23–41	32,563
October 22	2:00 p.m.	at Oklahoma State	Lewis Field Stillwater, OK		T 31–31	36,310
October 29	1:00 p.m.	Missouri	Cyclone Stadium Ames, IA (rivalry)		L 20–34	31,530
November 5	1:10 p.m.	at No. 15 Kansas State	KSU Stadium Manhattan, KS (rivalry)		L 20–38	38,572
November 12	1:00 p.m.	No. 1 Nebraska	Cyclone Stadium Ames, IA		L 12–28	45,186
November 19	1:00 p.m.	at No. 7 Colorado	Folsom Field Boulder, CO	PSN	L 20–41	46,113
*Non-conference game Homecoming Rankings from AP Poll released prior to the game All times are in Central time

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