The 1990 All-Big Eight Conference football team consists of American football players chosen by various organizations for All-Big Eight Conference teams for the 1990 NCAA Division I-A football season. The selectors for the 1990 season included the Associated Press (AP).

• Darian Hagan, Colorado (AP-1)
• Carl Straw, Kansas State (AP-2)

• Eric Bieniemy, Colorado (AP-1)
• Gerald Hudson, Oklahoma State (AP-1)
• Leodis Flowers, Nebraska (AP-2)
• Blaise Bryant, Iowa State (AP-2)

• Johnny Mitchell, Nebraska (AP-1)
• Tim Bruton, Missouri (AP-2)

• Mike Pritchard, Colorado (AP-1)
• Linzy Collins, Missouri (AP-1)
• Frank Hernandez, Kansas State (AP-2)
• Michael Smith, Kansas State (AP-2)

• Jay Leeuwenburg, Colorado (AP-1)
• Chip Budde, Kansas (AP-2)

• Joe Garten, Colorado (AP-1)
• Mark Vander Poel, Colorado (AP-1)
• Tom Punt, Nebraska (AP-1)
• Gene Williams, Iowa State (AP-1)
• Will Shields, Nebraska (AP-2)
• Mike Sawatzky, Oklahoma (AP-2)
• Jim Wanek, Nebraska (AP-2)
• Chris Perez, Kansas (AP-2)

• Kanavis McGhee, Colorado (AP-1)
• Alfred Williams, Colorado (AP-1)
• Jason Gildon, Oklahoma State (AP-2)
• James Goode, Oklahoma (AP-2)

• Kenny Walker, Nebraska (AP-1)
• Joel Steed, Colorado (AP-1)
• Scott Evans, Oklahoma (AP-1)
• Garry Howe, Colorado (AP-1)
• Stacey Satterwhite, Oklahoma State (AP-2)
• Gilbert Brown, Kansas (AP-2)
• Pat Engelbert, Nebraska (AP-2)

• Mike Croel, Nebraska (AP-1)
• Pat Tyrance, Nebraska (AP-1)
• Chris Wilson, Oklahoma (AP-2)
• Brooks Barta, Kansas State (AP-2)
• Joe Bowden, Oklahoma (AP-2)

• Reggie Cooper, Nebraska (AP-1)
• Harry Colon, Missouri (AP-1)
• Tim James, Colorado (AP-1)
• Mike Clark, Oklahoma State (AP-2)
• Bruce Pickens, Nebraska (AP-2)
• Marcus Robertson, Iowa State (AP-2)

• Jeff Shudak, Iowa State (AP-1)
• Dan Eichoff, Kansas (AP-2)

• Dan Eichoff, Kansas (AP-1)
• Cary Blanchard, Oklahoma State (AP-2)

AP = Associated Press^[1]

• 1990 College Football All-America Team