Events from the year 1539 in art.

• Hans Baldung – Virgin and Child
• Jacopo Bassano – Christ Among the Doctors
• Hans Holbein the Younger – Portrait of Anne of Cleves (probable date)

• June 13 - Jost Amman, Swiss illustrator (died 1591)
• Pietro Facchetti, Italian painter primarily of portraits (died 1613)
• Hasegawa Tōhaku, Japanese painter and founder of the Hasegawa school of Japanese painting during the Azuchi-Momoyama period (died 1610)

• Isabella d'Este, Italian noblewoman (Marchioness of Mantua), art collector, and patroness (born 1474)
• Marco Palmezzano, Italian painter and architect (born 1460)
• Il Pordenone, Italian painter of the Venetian school, active during the Renaissance (born 1483)
• Giacomo Pacchiarotti, Italian painter (born 1474)