TLR8 seems to function differently in humans and mice. Until recently, TLR8 was believed to be nonfunctional in mice, but it seems to counteract TLR7 activity[6][7]
The TLR family plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.[8] Recent research has also shown the expression of TLR8 in hippocampal interneurons, with yet unknown function.[9]
TLR8 can recognize GU-rich single-stranded RNA.[10] However, the presence of GU-rich sequences in the single-stranded RNA is not sufficient to stimulate TLR8.[11] TLR8 recognizes G-rich oligonucleotides.[12] TLR8 is activated by ssRNA and forms a dimer complex when uridine released from the degraded ssRNA binds at one active site in between the dimers and a short oligonucleotide binds to another active site on the surface of the TLR8 structure.[13]
TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza, Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits the myeloid differentiation primary response protein 88 (MyD88) and leads to activation of the transcription factor NF-κB and an antiviral response that leads to proinflammatory cytokine synthesis.[14][15] TLR8 recognizes single-stranded RNA of viruses such as HIV and HCV.[10][11] TLR8 is also involved in the activation of dendritic cells to produce inflammatory factors to help regulate tumor growth, so TLR8 is often used as a target in the research for therapies in treating cancers including ovarian cancer and lymphomas.[15]
^Seizer L, Rahimi S, Santos-Sierra S, Drexel M (2022) Expression of toll like receptor 8 (TLR8) in specific groups of mouse hippocampal interneurons. PLoS ONE 17(5): e0267860. https://doi.org/10.1371/journal.pone.0267860
^ abHuang X, Zhang X, Lu M (August 2021). "Recent trends in the development of Toll-like receptor 7/8-targeting therapeutics". Expert Opinion on Drug Discovery. 16 (8): 869–880. doi:10.1080/17460441.2021.1898369. PMID33678093. S2CID232141585.
