Priestley may refer to:

• Priestley, West Virginia, US, an unincorporated community
• Priestley Glacier, a major valley glacier in Antarctica
• Priestley (lunar crater), on the far side of the Moon
• Priestley (Martian crater)
• 5577 Priestley, an inner main belt asteroid

• Alice Priestley (born 1962), Canadian children's writer and illustrator
• Bel Priestley (born 2003), English actress and internet personality
• Brian Priestley (born 1940), English jazz writer, pianist and arranger
• Joanna Priestley (born 1950), American film director and animator
• Chris Priestley (born 1958), British children's book author and illustrator
• Jason Priestley (born 1969), Canadian-American actor
• J. B. Priestley (1894–1984), English writer and broadcaster
• Mark Priestley (1976–2008), Australian actor
• Rick Priestley (born 1959), British miniature wargame designer and author
• Robert Priestley (1901–1986), American set decorator
• Tom Priestley (born 1932), sound and film editor

• Henry Priestley (biochemist) (1884–1961), Australian biochemist
• Hilary Priestley, British mathematician
• James Taggart Priestley (1903–1979), American surgeon
• John Gillies Priestley (1879–1941), British physiologist
• Joseph Hubert Priestley (1883–1944), British botanist
• Joseph Priestley (1733–1804), British theologian, dissenting clergyman, natural philosopher, chemist, educator, and political theorist
• Maurice Priestley (1933–2013), emeritus professor of statistics at the University of Manchester
• Raymond Priestley (1886–1974), British geologist and Antarctic explorer

• Adam Priestley (born 1990), Gibraltarian footballer
• Akeem Priestley (born 1985), Jamaican footballer
• Dennis Priestley (born 1950), English darts player
• Donald Priestley (1887–1917), English cricketer
• Gerry Priestley (1931–2020), English footballer
• John Priestley (footballer) (1900–1980), Scottish footballer
• Neil Priestley (born 1961), English cricketer
• Robert Priestley (1911–2007), English cricketer
• Tommy Priestley (1911–1985), Northern Irish footballer

• Arthur Priestley (1865–1933), English Liberal Party politician and cricketer
• Briggs Priestley (1831–1907), English cloth manufacturer and Liberal Party politician
• Julian Priestley (1950–2017), Secretary-General of the European Parliament 1997–2007
• Mary Priestley (1925–2017), British music therapist
• Monique Priestley, American politician from Vermont
• Philip Priestley (1946–2022), British diplomat

• Priestley College, a sixth-form college
• The Priestley, a proscenium theatre
• Dr. Priestley, a fictional investigator

• Priestly, a surname