Phosphoric monoester hydrolases (or phosphomonoesterases) are enzymes that catalyse the hydrolysis of O-P bonds by nucleophilic attack of phosphorus by cysteine residues or coordinated metal ions.

They are categorized with the EC number 3.1.3.

Examples include:

• acid phosphatase
• alkaline phosphatase
• fructose-bisphosphatase
• glucose-6-phosphatase
• phosphofructokinase-2
• phosphoprotein phosphatase
• calcineurin
• 6-phytase

• phosphodiesterase
• phosphatase

• Phosphoric+Monoester+Hydrolases at the U.S. National Library of Medicine Medical Subject Headings (MeSH)