Grube is a surname. Notable people with the surname include:

• Adolph Eduard Grube (1812–1880), Polish zoologist
• Bruce Grube, 11th president of Georgia Southern University
• Charles Grube (1904–1976), American football player
• Chris Grube (born 1985), British sailor
• Christian Grube (born 1934), German conductor
• Ernst J. Grube (1932−2011), German historian of Islamic art and curator at the Metropolitan Museum of Art
• Frank Grube (1905–1945), American baseball player
• Franklin Grube (1831−1869), American physician and politician
• George Grube (1899–1982), classicist
• Henry Grube (died 1582), English politician
• Janice Grube, (born 1971), American singer-songwriter
• Jarrett Grube (born 1981), American baseball player
• Nikolai Grube, (born 1962), German epigrapher
• Rüdiger Grube (born 1951), engineer
• Wilfried Grube (born 1923), German former field hockey player
• Wilhelm Grube (1855–1908), German ethnographer