Dr. Mario is a 1990 action puzzle video game for the NES, Game Boy, and other Nintendo consoles.

Dr. Mario may also refer to:

In video games:

• Dr. Mario 64, a 2001 puzzle video game for the Nintendo 64.
• Dr. Mario Express, a 2008 DSiWare puzzle video game for the Nintendo DSi.
• Dr. Mario Online Rx, a 2008 WiiWare puzzle video game for the Nintendo Wii.
• Dr. Mario: Miracle Cure, a 2015 puzzle video game for the Nintendo 3DS.
• Dr. Mario World, a 2019 match-three game for mobile devices.

In medicine:

• Jorge Mario García Laguardia, also known as Dr. Jorge Mario García Laguardia, a Guatemalan jurist.
• Mario Costa (diplomat), also known as Dr. Mario Costa, the Republic of Malta Ambassador to the Russian Federation.
• Mario Díaz Martínez, also known as Dr. Mario Díaz Martínez, one of 12 elected volunteer members of the World Scout Committee.
• Mario Raviglione, also known as Dr. Mario Raviglione, Director of the Stop TB Department.

In sports:

• Estádio Martins Pereira, also known as Estádio Municipal Dr. Mário Martins Pereira, a football stadium in São José dos Campos, São Paulo.