Darnley may refer to:

• Henry Stuart, Lord Darnley, consort of Mary, Queen of Scots
• Earl of Darnley, peerage title

• Darnley, part of Glasgow, Scotland
• Darnley Island (Queensland), Queensland, Australia
• Two places in Antarctica
  • Cape Darnley (Mac. Robertson Land)
  • Cape Darnley (South Georgia)
• Two places in Canada
  • Darnley, Prince Edward Island
  • Darnley Bay, Northwest Territories

• Darnley, a fictional city in Philip George Chadwick's The Death Guard
• Darnley Mills, a fictional town in books by Philip Turner
• Darnley, or, The Field of the Cloth of Gold, an 1830 novel by George Payne Rainsford James

• Darnley Lime Works Tramway and Mineral Railway
• Dams to Darnley Country Park