BAF agar
BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi.[1] It was first described by A.J.P. Oort in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981.[1] The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.[2]
Typical composition
BAF agar typically contains:[2]
- 30.0 g/L glucose
- 2.0 g/L peptone
- 0.2 g/L yeast extract
- 0.5 g/L KH2PO4
- 0.5 g/L MgSO4.7 H2O
- 10.0 mg/L FeCl3.6 H2O
- 1.0 mg/L ZnSO4.7 H2O
- 5.0 mg/L MnSO4
- 100.0 mg/L CaCl2.2 H2O
- 50.0 μg/L thiamine HCl
- 1.0 μg/L biotin
- 100.0 μg/L folic acid
- 50.0 μg/L inositol
- 15 g/L agar
References
- ^ a b Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. Bibcode:2008Mycor..18..443A. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
- ^ a b "CBS List of Media" (PDF).