The 27th Annual Annie Awards were held on November 6, 1999.

Winners are marked in bold text.

• A Bug's Life
• The Iron Giant
• The Prince of Egypt
• South Park: Bigger, Longer & Uncut
• Tarzan

• The Land Before Time VI: The Secret of Saurus Rock
• The Lion King II: Simba's Pride
• Pocahontas II: Journey to a New World
• Scooby-Doo on Zombie Island

• The Goddamn George Liquor Program - Spumco, Inc.

• Al Tudi Tuhak
• Bunny
• Living Forever
• More

• Gnome – "Hollywood Gum"
• Kraft Foods – "Tweety"
• Levi Strauss & Co. – "Sensitive"
• San Francisco Film Society – "Celebrate Being in the Dark"
• Old Navy – "Performance Fleece"

• Batman Beyond
• Futurama
• King of the Hill
• The New Batman/Superman Adventures
• The Simpsons

• Peter De Mund – Tarzan – Walt Disney Pictures
• Allen Foster – The Iron Giant – Warner Bros. Feature Animation
• Michel Gagné – The Iron Giant – Warner Bros. Feature Animation
• Joel Krasnove – The Angry Beavers – Nickelodeon Animation Studio
• Jamie Lloyd – The Prince of Egypt – DreamWorks Pictures

• Ken Duncan – Tarzan – Walt Disney Pictures
• Glen Keane – Tarzan – Walt Disney Pictures
• Steve Markowski – The Iron Giant – Warner Bros. Feature Animation
• Jim van der Keyl – The Iron Giant – Warner Bros. Feature Animation
• Dean Wellins – The Iron Giant – Warner Bros. Feature Animation

• Brad Bird – The Iron Giant – Warner Bros. Feature Animation
• Brenda Chapman, Steve Hickner and Simon Wells – The Prince of Egypt – DreamWorks Pictures
• Eric Darnell and Tim Johnson – Antz – DreamWorks Pictures and PDI
• John Lasseter and Andrew Stanton – A Bug's Life – Walt Disney Pictures and Pixar Animation Studios
• Kevin Lima and Chris Buck – Tarzan – Walt Disney Pictures

• Mark Gustafson – "Bougie Nights", The PJs – Will Vinton Studios
• John McIntyre – "Mommie Fearest", The Powerpuff Girls – Cartoon Network Studios
• Nelson Recinos – "How I Spent My Weekend", Steven Spielberg Presents Pinky, Elmyra & the Brain – Warner Bros. Television Animation
• Dave Wasson – "Max & His Special Problem", Oh Yeah! Cartoons – Nickelodeon Animation Studio

• Phil Collins (Composer and Lyricist) – "Two Worlds" (Song), Tarzan – Walt Disney Pictures
• Harry Gregson-Williams and John Powell (Composers) – Antz – DreamWorks Pictures and PDI
• Michael Kamen (Composer) – The Iron Giant – Warner Bros. Feature Animation
• Tom Snow (Composer), Marty Panzer and Jack Feldman (Lyricists) – "We Are One" (Song), The Lion King II: Simba's Pride – Walt Disney Television Animation
• Scott Warrender (Composer) and Joss Whedon (Lyricist) – "My Lullaby" (Song), The Lion King II: Simba's Pride – Walt Disney Television Animation

• Walter Murphy (Composer), Seth MacFarlane and David Zuckerman (Lyricists) – Main Title, Family Guy – 20th Century Fox Television
• Randy Rogel (Composer and Lyricist) – "That Is the Story That's Told by the Bard" (Song), Histeria! – Warner Bros. Television Animation
• J. Eric Schmidt and Cameron Patrick (Composers) – The San Francisco Beat (Episode), The Sylvester and Tweety Mysteries – Warner Bros. Television Animation
• Stephen James Taylor (Composer) – "Pluto's Arrow Error" (Episode), Mickey Mouse Works – Walt Disney Television Animation

• John Bell – Antz – DreamWorks Pictures and PDI
• Alan Bodner – The Iron Giant – Warner Bros. Feature Animation
• William Cone – A Bug's Life – Walt Disney Pictures and Pixar Animation Studios
• Daniel St. Pierre – Tarzan – Walt Disney Pictures
• Mark Whiting – The Iron Giant – Warner Bros. Feature Animation

• Paul Harrod – "Boyz 'N' The Woods", The PJs – Will Vinton Studios
• Craig Kellman – "Uh Oh Dynamo", The Powerpuff Girls – Cartoon Network Studios
• Glen Murakami – "Legends of the Dark Knight", The New Batman/Superman Adventures – Warner Bros. Television Animation
• Sue Rose – "Friday", 4WD (Four Women Driving) – Walt Disney Television Animation
• Jay Stephens – "Sacred Identity", JetCat – Porchlight Entertainment

• Mark Andrews – The Iron Giant – Warner Bros. Feature Animation
• Lorna Cook – The Prince of Egypt – DreamWorks Pictures
• Kevin O'Brien – The Iron Giant – Warner Bros. Feature Animation
• Brian Pimental – Tarzan – Walt Disney Pictures
• Dean Wellins – The Iron Giant – Warner Bros. Feature Animation

• Alex Kirwan – "The Man with No Nose", Oh Yeah! Cartoons – Nickelodeon Animation Studio
• Bob McKnight – "William Tell Overture", Mickey Mouse Works – Walt Disney Television Animation
• Mitch Schauer – "The Day the World Got Really Screwed Up", The Angry Beavers – Nickelodeon Animation Studio
• Adam Van Wyk – "Black Out", Batman Beyond – Warner Bros. Television Animation
• Dave Wasson – "Max & His Special Problem", Oh Yeah! Cartoons – Nickelodeon Animation Studio

• Mary Kay Bergman – voice of Sheila Broflovski – South Park: Bigger, Longer & Uncut – Paramount Pictures
• Minnie Driver – voice of Jane – Tarzan – Walt Disney Pictures
• Ralph Fiennes – voice of Rameses – The Prince of Egypt – DreamWorks Pictures
• Eli Marienthal – voice of Hogarth Hughes – The Iron Giant – Warner Bros. Feature Animation
• Suzanne Pleshette – voice of Zira – The Lion King II: Simba's Pride – Walt Disney Television Animation

• Charlie Adler – voice of Cow – Cow and Chicken – Cartoon Network Studios
• Tara Charendoff – voice of Bubbles – The Powerpuff Girls – Cartoon Network Studios
• Eddie Murphy – voice of Thurgood Stubbs – The PJs – Will Vinton Studios
• Rob Paulsen – voice of Pinky – Steven Spielberg Presents Pinky, Elmyra & The Brain – Warner Bros. Television Animation
• Cree Summer – voice of Elmyra – Steven Spielberg Presents Pinky, Elmyra & The Brain – Warner Bros. Television Animation

• Todd Alcott, Chris Weitz and Paul Weitz – Antz – DreamWorks Pictures and PDI
• Brad Bird and Tim McCanlies – The Iron Giant – Warner Bros. Feature Animation
• John Lasseter, Andrew Stanton, Joe Ranft, Donald McEnery and Bob Shaw – A Bug's Life – Walt Disney Pictures and Pixar Animation Studio.
• Tab Murphy, Bob Tzudiker and Noni White – Tarzan – Walt Disney Pictures
• Trey Parker, Matt Stone and Pam Brady – South Park: Bigger, Longer & Uncut – Paramount Pictures

• Alan Burnett and Paul Dini – "Rebirth Part I", Batman Beyond – Warner Bros. Television Animation
• Jim Dauterive – "Hank's Cowboy Movie", King of the Hill – 20th Century Fox Television in association with Deedle-Dee Productions, Judgemental Films, and 3 Arts Entertainment
• Evan Dorkin and Sarah Dyer – "Lawsuit", Space Ghost Coast to Coast – Cartoon Network Productions, Inc.
• Ken Keeler – "The Series Has Landed", Futurama – The Curiosity Co. in association with 20th Century Fox Television
• Tim Long, Larry Doyle and Matt Selman – "Simpsons Bible Stories", The Simpsons – Gracie Films in association with 20th Century Fox Television

Significant and benevolent or charitable impact on the art and industry of animation.

• Dave Master

Recognition of lifetime or career contributions to the art of animation.

• Marcell Jankovics
• Ray Patterson
• Ernie Con Pederson

• Eric Daniels

• Melanie Crandall
• Michael Mallory

• Official website
• 27th Annie Awards at IMDb