Margaret Hamilton may refer to:

• Margaret Hamilton (nurse) (1840–1922), American nurse in the Civil War
• Maggie Hamilton (1867–1952), Scottish artist
• Margaret Hamilton (educator) (1871–1969), American educator
• Margaret Hamilton (actress) (1902–1985), American film character actress
• Margaret Hamilton (software engineer) (born 1936), American software engineer
• Margaret Hamilton (publisher) (1941–2022), Australian publisher of children's literature