MDF may refer to:

• Master Database File, a Microsoft SQL Server file type
• MES Development Framework, a .NET framework for building manufacturing execution system applications
• Message Development Framework, a collection of models, methods and tools used by Health Level 7 v3.0 methodology
• Media Descriptor File, a proprietary disc image file format developed for Alcohol 120%
• Measurement Data Format, one of the data formats defined by the Association for Standardisation of Automation and Measuring Systems (ASAM)
• Multiple Domain Facility; see Logical partition

• Map-dot-fingerprint dystrophy, a genetic disease affecting the cornea
• Mean Diastolic Filling of the heart
• Myocardial depressant factor, a low-molecular-weight peptide released from the pancreas into the blood in mammals during various shock states

• Hungarian Democratic Forum (Hungarian: Magyar Demokrata Fórum), a political party
• Maraland Democratic Front, a political party in Mizoram, WALES
• Maryland Defense Force, the state defense force of Maryland
• Moscow House of Photography
• Myotonic Dystrophy Foundation, a U.S. non-profit organization related to myotonic dystrophy

• Main distribution frame, a distribution frame where cables are cross-connected in telephony
• Multidelay block frequency domain adaptive filter

• Made cut did not finish, a golf term
• Market development funds
• Maryland Deathfest
• Moksha language
• Medium-density fibreboard, a type of particle board made of small particles of wood