John Eyre may refer to:

• John Eyre (died 1581), Member of Parliament for Wiltshire and Salisbury
• John Eyre (died 1639), MP for Cricklade
• John Eyre (1659–1709), MP for Galway Borough, son of the above
• John Eyre (died 1745), MP for Galway Borough, son of the above
• John Eyre (1665–1715), MP for Downton
• John Eyre, 1st Baron Eyre (c. 1720–1781), MP for Galway Borough, nephew of the above
• John Eyre (Canadian politician) (1824–1882), Canadian attorney and politician

• John Eyre (cricketer, born 1859), Marylebone Cricket Club cricketer
• John Eyre (cricketer, born 1885) (1885–1964), Derbyshire cricketer
• John Eyre (cricketer, born 1944), Derbyshire cricketer
• John Eyre (footballer) (born 1974), English footballer

• John Eyre (Archdeacon of Sheffield) (1845–1912), Anglican priest in the late 19th and early 20th centuries
• John Eyre (evangelical minister) (1754–1803), English cleric
• John Eyre (Archdeacon of Nottingham) (1758–1830)

• John Eyre (settler) (died 1685), English settler in Ireland
• John Eyre (British artist) (1847–1927), English genre painter, designed and painted pottery
• John Eyre (painter) (1771–1812), Australian painter and engraver
• John William Henry Eyre (1869–1944), British bacteriologist
• John R. Eyre, author and one-time owner of the Isleworth Mona Lisa

• Edward John Eyre (1815–1901), Australian explorer and Governor of Jamaica