Beef is the meat from cattle.

Beef may also refer to:

• Beef (Nitro Girl), a stage name of professional wrestler Rhonda Sing with the Nitro Girls
• Beef (rapper), American rapper
• "Beef", nickname of English golfer Andrew Johnston

• Beef (band), a Dutch reggae fusion band
• Beef (comics), a character in the Marvel universe
• Beef (film), a 2003 documentary film about hip hop feuds, also called beefs
• Beef (TV series), a 2023 Netflix comedy drama series
  • Beef (soundtrack), the 2023 soundtrack album of the series
• "Beef" (The Bear), a second-season episode of The Bear
• "Beef" (Law & Order: Special Victims Unit), an eleventh-season episode of Law & Order: Special Victims Unit

• "Beef", quarrying term for fibrous calcite veins found within some types of mudstones
• "Beef", the primary essential part of a thing, the absence of which could induce the question, "Where's the beef?"
• Beef Creek, a stream in South Dakota
• Big Explosives Experimental Facility
• British Energy Efficiency Federation