USS Gladiator may refer to:

• USS Gladiator (1876), a tugboat, built 1876, in service briefly with the U.S. Navy in 1918
• USS Gladiator (AM-319), a U.S. Navy minesweeper commissioned 1944, active during World War II, decommissioned 1955
• USS Gladiator (MCM-11), an Avenger-class mine countermeasures ship of the U.S. Navy, commissioned 1993