St. James Hotel may refer to:

• St. James Hotel (Jacksonville, Florida), a destroyed hotel, the site of the St. James Building
• St. James Hotel (Red Wing, Minnesota), listed on the National Register of Historic Places (NRHP)
• St. James Hotel (Cimarron, New Mexico)
• St. James Hotel (Philadelphia), listed on the NRHP
• St. James Hotel (San Diego), part of the Gaslamp Quarter Historic District
• St James's Hotel and Club, London