Punchline is a supervillain who appears in American comic books published by DC Comics, commonly in association with the supervillain The Joker. Created by James Tynion IV and Jorge Jiménez, she first appears in Batman (vol. 3) #89 (April 2020), during the Joker War story arc.^[1][2][3][4]

Debuting shortly before The Joker War story arc, Alexis Kaye is a college student who develops an unhealthy obsession with the Joker, after encountering him during a grisly-fated high school trip. Facing the death penalty for the crime she committed over the recent times, Kaye pleads not guilty. Being released from court, she develops a plot to become The Joker's new sidekick and girlfriend.

In 2022, the character was given a limited series, titled Punchline: The Gotham Game, in which she moves on from The Joker and becomes the new leader of Gotham's Royal Flush Gang. The title ran for 6 issues, and was written by Tini Howard, with art provided by Blake Howard, Gleb Melnikov.^[5]

In the Knight Terrors story arc, Punchline and the Royal Flush Gang attempt to break into the supposedly abandoned Clock Tower that was used by the Bat Family. With a large number of heroes in the Knightmare Realm, she believes that the process will be an easy task before coming face-to-face with her deepest fear.^[6]

Unlike the majority of DC villains, Punchline has no metahuman powers or abilities. However, she is adept at wielding knives and makes use of Joker venom, along with other toxins.