Protein-coding gene in the species Homo sapiens
Tyrosine-protein phosphatase non-receptor type 12 is an enzyme that in humans is encoded by the PTPN12 gene .[ 5] [ 6]
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.[ 6]
Interactions PTPN12 has been shown to interact with BCAR1 ,[ 7] [ 8] [ 9] [ 10] Grb2 ,[ 11] PSTPIP1 ,[ 12] TGFB1I1 ,[ 13] Paxillin [ 14] [ 15] [ 16] SHC1 .[ 17] [ 18]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, Imai K, Yachi A (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Lett . 339 (3): 222–8. doi :10.1016/0014-5793(94)80420-6 . PMID 7509295 . S2CID 4885570 . ^ a b "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12" .^ Lin, Yi; Ceacareanu Alice Corina; Hassid Aviv (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". Am. J. Physiol. Heart Circ. Physiol . 285 (2): H710–21. doi :10.1152/ajpheart.01127.2002 . ISSN 0363-6135 . PMID 12714323 . S2CID 42587789 . ^ Garton, A J; Burnham M R; Bouton A H; Tonks N K (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition" . Oncogene . 15 (8): 877–85. doi :10.1038/sj.onc.1201279 ISSN 0950-9232 . PMID 9285683 . ^ Côté, J F; Charest A; Wagner J; Tremblay M L (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry . 37 (38): 13128–37. doi :10.1021/bi981259l . ISSN 0006-2960 . PMID 9748319 . ^ Garton, A J; Flint A J; Tonks N K (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST" . Mol. Cell. Biol . 16 (11): 6408–18. doi :10.1128/MCB.16.11.6408 . ISSN 0270-7306 . PMC 231642 PMID 8887669 . ^ Charest, A; Wagner J; Kwan M; Tremblay M L (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2" . Oncogene . 14 (14): 1643–51. doi :10.1038/sj.onc.1201008 ISSN 0950-9232 . PMID 9135065 . ^ Dowbenko, D; Spencer S; Quan C; Lasky L A (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein" . J. Biol. Chem . 273 (2): 989–96. doi :10.1074/jbc.273.2.989 ISSN 0021-9258 . PMID 9422760 . ^ Nishiya, N; Iwabuchi Y; Shibanuma M; Côté J F; Tremblay M L; Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain" . J. Biol. Chem . 274 (14): 9847–53. doi :10.1074/jbc.274.14.9847 ISSN 0021-9258 . PMID 10092676 . ^ Shen, Y; Lyons P; Cooley M; Davidson D; Veillette A; Salgia R; Griffin J D; Schaller M D (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo" . J. Biol. Chem . 275 (2): 1405–13. doi :10.1074/jbc.275.2.1405 ISSN 0021-9258 . PMID 10625692 . ^ Côté, J F; Turner C E; Tremblay M L (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST" . J. Biol. Chem . 274 (29): 20550–60. doi :10.1074/jbc.274.29.20550 ISSN 0021-9258 . PMID 10400685 . ^ Shen, Y; Schneider G; Cloutier J F; Veillette A; Schaller M D (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin" . J. Biol. Chem . 273 (11): 6474–81. doi :10.1074/jbc.273.11.6474 ISSN 0021-9258 . PMID 9497381 . ^ Habib, T; Herrera R; Decker S J (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase" . J. Biol. Chem . 269 (41): 25243–6. doi :10.1016/S0021-9258(18)47237-7 ISSN 0021-9258 . PMID 7929214 . ^ Charest, A; Wagner J; Jacob S; McGlade C J; Tremblay M L (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity" . J. Biol. Chem . 271 (14): 8424–9. doi :10.1074/jbc.271.14.8424 ISSN 0021-9258 . PMID 8626541 .
Further reading
Takekawa M, Itoh F, Hinoda Y, et al. (1993). "Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase". Biochem. Biophys. Res. Commun . 189 (2): 1223–30. doi :10.1016/0006-291X(92)92335-U . PMID 1472029 . Yi T, Cleveland JL, Ihle JN (1991). "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification" . Blood . 78 (9): 2222–8. doi :10.1182/blood.V78.9.2222.2222 PMID 1932742 . Garton AJ, Tonks NK (1994). "PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation" . EMBO J . 13 (16): 3763–71. doi :10.1002/j.1460-2075.1994.tb06687.x . PMC 395289 PMID 7520867 . Habib T, Herrera R, Decker SJ (1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase" . J. Biol. Chem . 269 (41): 25243–6. doi :10.1016/S0021-9258(18)47237-7 PMID 7929214 . Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase" . J. Biol. Chem . 268 (23): 17650. doi :10.1016/S0021-9258(19)85383-8 PMID 8349645 . Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase" . J. Biol. Chem . 268 (9): 6622–8. doi :10.1016/S0021-9258(18)53296-8 PMID 8454633 . Charest A, Wagner J, Jacob S, et al. (1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity" . J. Biol. Chem . 271 (14): 8424–9. doi :10.1074/jbc.271.14.8424 PMID 8626541 . Garton AJ, Flint AJ, Tonks NK (1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST" . Mol. Cell. Biol . 16 (11): 6408–18. doi :10.1128/MCB.16.11.6408 . PMC 231642 PMID 8887669 . Charest A, Wagner J, Kwan M, Tremblay ML (1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2" . Oncogene . 14 (14): 1643–51. doi :10.1038/sj.onc.1201008 PMID 9135065 . Garton AJ, Burnham MR, Bouton AH, Tonks NK (1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition" . Oncogene . 15 (8): 877–85. doi :10.1038/sj.onc.1201279 PMID 9285683 . Dowbenko D, Spencer S, Quan C, Lasky LA (1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein" . J. Biol. Chem . 273 (2): 989–96. doi :10.1074/jbc.273.2.989 PMID 9422760 . Shen Y, Schneider G, Cloutier JF, et al. (1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin" . J. Biol. Chem . 273 (11): 6474–81. doi :10.1074/jbc.273.11.6474 PMID 9497381 . Côté JF, Charest A, Wagner J, Tremblay ML (1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry . 37 (38): 13128–37. doi :10.1021/bi981259l . PMID 9748319 . Wu Y, Dowbenko D, Lasky LA (1998). "PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated protein that binds a PEST-type protein-tyrosine phosphatase" . J. Biol. Chem . 273 (46): 30487–96. doi :10.1074/jbc.273.46.30487 PMID 9804817 . Sanger Centre, The; Washington University Genome Sequencing Cente, The (1999). "Toward a complete human genome sequence" . Genome Res . 8 (11): 1097–108. doi :10.1101/gr.8.11.1097 PMID 9847074 . Li J, Nishizawa K, An W, et al. (1999). "A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion" . EMBO J . 17 (24): 7320–36. doi :10.1093/emboj/17.24.7320 . PMC 1171078 PMID 9857189 . Garton AJ, Tonks NK (1999). "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST" . J. Biol. Chem . 274 (6): 3811–8. doi :10.1074/jbc.274.6.3811 PMID 9920935 . Angers-Loustau A, Côté JF, Charest A, et al. (1999). "Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts" . J. Cell Biol . 144 (5): 1019–31. doi :10.1083/jcb.144.5.1019 . PMC 2148201 PMID 10085298 . Nishiya N, Iwabuchi Y, Shibanuma M, et al. (1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain" . J. Biol. Chem . 274 (14): 9847–53. doi :10.1074/jbc.274.14.9847 PMID 10092676 .
