Karl of Mecklenburg-Güstrow
Hereditary Prince of Mecklenburg-Güstrow
Portrait by Gedeon Romandon
Born	(1664-11-18)18 November 1664 Güstrow
Died	15 March 1688(1688-03-15) (aged 23) Güstrow
Spouse	Marie Amalie of Brandenburg
House	House of Mecklenburg
Father	Gustav Adolph, Duke of Mecklenburg-Güstrow
Mother	Magdalene Sibylle of Holstein-Gottorp

Karl, Hereditary Prince of Mecklenburg-Güstrow (18 November 1664 in Güstrow – 15 March 1688 in Güstrow) was the hereditary prince of Mecklenburg-Güstrow. He was a son of Gustavus Adolph and his wife Magdalene Sibylle née Duchess of Schleswig-Holstein-Gottorp, a daughter of Frederick III.

Charles married on 10 August 1687 in Potsdam to Marie Amalie of Brandenburg, the daughter of the "Great Elector" Frederick William of Brandenburg. The marriage remained childless. He died unexpectedly of smallpox. He was the last surviving son of his father, and his wife lost her child when she learned of his fate, so that the Mecklenburg-Güstrow line died out when his father died in 1695.

His brother-in-law, Duke Adolf Frederick II of Mecklenburg-Strelitz claimed Mecklenburg-Güstrow. However Duke Frederick William of Mecklenburg-Schwerin disagreed and the legal situation was unclear, leading to a long succession dispute. The dispute was settled in the Partition of Hamburg, in which the relationship between Mecklenburg-Schwerin and Mecklenburg-Strelitz was redefined and Mecklenburg-Güstrow was given to Mecklenburg-Schwerin.

• Literature about Karl, Hereditary Prince of Mecklenburg-Güstrow in the State Bibliography (Landesbibliographie) of Mecklenburg-Vorpommern
• Publications by or about Karl, Hereditary Prince of Mecklenburg-Güstrow at VD 17
• Stammtafel des Hauses Mecklenburg