Ghirlandaio is the surname of a family of Renaissance Italian painters:

• Domenico Ghirlandaio (1449–1494), painter of fresco cycles and Michelangelo's teacher
• Davide Ghirlandaio (1452–1525), younger brother of Domenico
• Benedetto Ghirlandaio (1458–1497), younger brother of Domenico and Davide
• Ridolfo Ghirlandaio (1483–1561), son of Domenico

• Michele Tosini (1503–1577), Italian painter also known as Ghirlandaio