Deadly Nightshade may refer to:

• Deadly nightshade (Atropa bella-donna), a poisonous perennial herbaceous plant in the nightshade family
• Deadly Nightshade (comics), a Marvel Comics character
• Deadly Nightshade (film), a 1953 British crime drama film
• The Deadly Nightshade, an American rock and country band
• "Deadly Nightshade", a song by the Sword from the album Used Future

• Nightshade (disambiguation)