Twenty-Five or 25 may refer to:

• 25 (number)
• one of the years 25 BC, AD 25, 1925, 2025

• Manganese, a transition metal in the periodic table
• 25 Phocaea, an asteroid in the asteroid belt

• Twenty Five (album), a 2006 greatest hits album by George Michael
  • Twenty Five (video), released alongside the George Michael album
• 25 (A-ha album), 2010
• 25 (Adele album), 2015
• 25 (Blues Traveler album), 2012
• 25 (G Herbo album), 2021
• 25 (Harry Connick, Jr. album), 1992
• 25 (Oysterband album), 2003
• 25 (Patty Larkin album), 2010
• 25 (EP), a 2014 EP by Song Ji-eun
• Mina 25, a 1983 album by Mina

• "25" (Rod Wave song)
• "25", a song by The Kooks from 10 Tracks to Echo in the Dark (2022)
• "25", a song by The Pretty Reckless from Death by Rock and Roll, 2021
• "25", a song by The Smith Street Band from More Scared of You Than You Are of Me, 2017
• "Twenty Five", a song by Karma to Burn from the album Wild, Wonderful Purgatory, 1999

• Twenty-five (card game), the Irish national card game
• "Twenty Five" (The West Wing), a 2003 episode of the television series The West Wing
• Renault 25, an executive liftback
• Rover 25, a hatchback

• List of highways numbered 25