Events from the year 1541 in art.

• Michelangelo completes his fresco The Last Judgment on the altar wall of the Sistine Chapel in Vatican City.
• Cardinal Alexander writes to Bishop Marco Vigerio discussing frescoes to be done by Michelangelo for the Cappella Paolina.

• Battista Franco Veneziano paints fresco of the Arrest of John the Baptist for the Oratory of San Giovanni Decollato
• Pietro Negroni paints Adoration of Magi
• Titian – The Marquis of Vasto addressing his troops
• Antoine Caron – Dionysius Areopagite and the Eclipse of Sun^(image)

date unknown

• El Greco (or Domenikos Theotokopoulos), painter, sculptor, and architect of the Spanish Renaissance (died 1614)
• Aert Mijtens, Flemish Renaissance painter (died 1602)
• Yi Chong, Korean painter (died 1622)

• January 6 - Bernard van Orley, Flemish Northern Renaissance painter and draughtsman (born 1487-1491)
• August - Valentin Bousch, glass painter (born 1490)
• date unknown
  • Jean Clouet, miniaturist and painter who worked in France during the Renaissance (born 1480)
  • Gerard Horenbout, Flemish miniaturist (born 1465)
  • Stanisław Samostrzelnik, Polish Renaissance painter and Cistercian monk (born 1490)